This list only includes recurring characters, otherwise known as supporting characters, which appear frequently from time to time during the series' run, often playing major roles in more than one episode, and those in the main cast are listed below. Anime such as Adachi and Shimamura, Assault Lily Bouquet, My Next Life as a Villainess: All Routes Lead to Doom!, and Otherside Picnic also featured LGBTQ characters.

This includes series such as Kipo and the Age of Wonderbeasts, The Owl House, Helluva Boss, Star Trek: Lower Decks, Adventure Time: Distant Lands, and The Great North. The 2020s saw a blossoming of LGBTQ characters in animated series, including in children's animation, adult animation, young adult animation, and anime. You can help by adding missing items with reliable sources.

This is a dynamic list and may never be able to satisfy particular standards for completeness.